In vitro culture initiation and regeneration of two highly productive clones of poplar

In vitro shoot regeneration is a fast and reliable method for propagation of valuable tree genotypes important step in genetic manipulations. This study aimed to establish an optimal culture initiation protocol highly productive poplar clones ‘Novoberlinska-3’ (Populus pyramidalis × P. laurifolia) ‘Volosystoplidna’ (P. trichocarpa). was initiated either on MS (IM-MS) or WPM (IM-WPM) medium both containing BAP (0.2 mg.L-1) IBA (0.1 mg.L-1). Results demonstrated that the best clone IM-WPM which 93.3 % explants survived, while from better survived IM-MS. After establishing into aseptic culture, experiments were started using two types explants, leaf petioles, planted callus induction 2iP (1.02 NAA (1.86 Furthermore, microshoots placed supplemented with 0.04 mg.L-1 thidiazuron, then transferred elongation 0.2 BAP. Regeneration showed performance ‘Novoberlinska-3’, but it also efficient ‘Volosystoplidna’. Plant highest percentage (92.3 %) number shoots per explant (3.1), significantly exceeded other explants. Our results significant difference between survivability during initiation. The differences rates as well petiole determined. Obtained cultures hybrids will be used our further studies, especially transformation.